Coordination of mitochondrial bioenergetics with G1phase cell cycle progression

Stefan M. Schieke, J. Philip McCoy Jr., Toren Finkel
2008 Cell Cycle  
Relatively little is known regarding how energetic demand during cell proliferation is sensed or coordinated with mitochondrial metabolism. Here we demonstrate that cell cycle progression through is associated with a significant increase in mitochon-G 1 drial membrane potential (ΔΨ m ) and respiration. We used this with low and high change in metabolic rate to isolate cells in G 1 levels of mitochondrial membrane potential (ΔΨ m L and ΔΨ m H). Biochemical and functional studies demonstrate that
more » ... ΔΨ m L and ΔΨ m H cells display the distinct characteristics of early and late phase, respectively. We further demonstrate that the metabolic G 1 rate in reflects levels of the mTOR-raptor complex as well as G 1 susceptibility to rapamycin-induced cell cycle delay. In conclusion, our data suggests a coupling of mitochondrial bioenergetics and progression and points to the mTOR signaling pathway as a G 1 potential molecular coordinator of these two processes.
doi:10.4161/cc.7.12.6067 pmid:18583942 pmcid:PMC3399174 fatcat:zkyum52drnh5pk2jzuq5cped2e