Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound

Jae-Jung Lee, Jun-Young Kim, Duanting Zhai, Seong-Wook Yun, Young-Tae Chang
2012 Interdisciplinary Bio Central  
These authors contributed equally to this work Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Westernblotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent
more » ... ical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.
doi:10.4051/ibc.2012.4.2.0005 fatcat:oieic6rebrfidcjzko6u44yizq