Identification of candidate genes for lung cancer somatic mutation test kits

Yong Chen, Jian-Xin Shi, Xu-Feng Pan, Jian Feng, Heng Zhao
2013 Genetics and Molecular Biology  
Over the past three decades, mortality from lung cancer has sharply and continuously increased in China, ascending to the first cause of death among all types of cancer. The ability to identify the actual sequence of gene mutations may help doctors determine which mutations lead to precancerous lesions and which produce invasive carcinomas, especially using next-generation sequencing (NGS) technology. In this study, we analyzed the latest lung cancer data in the COSMIC database, in order to
more » ... genomic "hotspots" that are frequently mutated in human lung cancer genomes. The results revealed that the most frequently mutated lung cancer genes are EGFR, KRAS and TP53. In recent years, EGFR and KRAS lung cancer test kits have been utilized for detecting lung cancer patients, but they presented many disadvantages, as they proved to be of low sensitivity, labor-intensive and time-consuming. In this study, we constructed a more complete catalogue of lung cancer mutation events including 145 mutated genes. With the genes of this list it may be feasible to develop a NGS kit for lung cancer mutation detection.
doi:10.1590/s1415-47572013000300022 pmid:24130455 pmcid:PMC3795175 fatcat:7zbabhbwmjexrmm3bpxown6mty