In isolated rabbit urinary bladder preparations, Artemisia vulgaris (Av.Cr) crude extract inhibited the contractions produced by carbachol (CCh, 1 µM) and high K + (80 mM) in a concentration-dependent (0.03-10 mg/mL) manners. The crude extract of Aspalathus linaeris (Al. Cr) relaxed the contractions produced by low K + (25 mM) at concentration range of 0.3-1.0 mg/mL, having moderate inhibitory effect on contractions produced by K + (80 mM). Contractions induced by low K + were partially

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... zed by glibenclamide (3 µM). These results indicate that urinary bladder relaxation produced by Artemisia vulgaris extract is mediated via dual, anticholinergic and Ca ++ antagonist mechanisms, while that of Aspalathus linaris via dominant KATP channel opening and weak Ca ++ channel blockade pathways. Thus the present study reveals the Artemisia vulgaris and Aspslathus linaeris medicinal usefulness in hyperactive bladder disorders