Sophisticated analytical methods viz. Spectroscopic methods (A and B) which being employed for analysis, are relatively expensive and hence need for simple analytical methods arises. Such methods has been developed and applied for routine determination of riboflavin in pharmaceutical formulations and bulk dosage forms. These methods were based on the formation of colored species via reaction of the enzyme amylase phosphate with Ferric chloride, n-methyl benzothiazolone hydra zone (MBTH),

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... um ferrocyanide in the presence of HCl and finally with the drug to be estimated namely riboflavin to produce light green and blue colored chromogen (λmax at 430 and 700nm). Statistical analysis of these methods exhibited a molar absorptivity value 10