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A Method of Primary Cell Culture for Establishing Equine Long-Term Culture Cell Lines
Journal of Equine Science
We investigated a method of culture for the establishment of equine long-term culture cell lines. The tissues tested were equine fetus tissues; right abdomen epithelium, the umbilical artery, the umbilical ring, the pulmonary artery, the renal cortex and the fetal placenta. To decide on a suitable primary culture method, we compared two methods of primary culture: tissue fragment culture and low-temperature trypsin cell-dispersal. On the other hand, to decide on a suitable culture medium, wedoi:10.1294/jes.8.95 fatcat:goo3vy5lincu7kz6dvontdg6qm