Viability of dourado embryos cooled in different cryoprotectant solutions

Darci Carlos Fornari, Danilo Pedro Streit Junior, Juliana Minardi Galo, Luís Jayme Guerreiro, Jayme Aparecido Povh, Nelson Mauricio Lopera Barrero, Diego de Oliveira, Ricardo Pereira Ribeiro
2013 Pesquisa Agropecuária Brasileira  
The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (-8ºC for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution
more » ... solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.
doi:10.1590/s0100-204x2013000800057 fatcat:fuedasyqqnbh5f5nsix4jr6sdy