Inhibition of Rickettsia conorii growth by recombinant tumor necrosis factor alpha: enhancement of inhibition by gamma interferon

E Manor, I Sarov
1990 Infection and Immunity  
Purified human recombinant tumor necrosis factor alpha (rTNF-a) inhibited the growth of Rickeffsia conorii (Casablanca strain) in HEp-2 cell culture. The effect was observed when the cells were pretreated with rTNF-at or when rTNF-a was added after adsorption of the rickettsiae. The inhibitory effect of rTNF-oa on rickettsial growth was enhanced by gamma interferon. Cycloheximide had no effect on inhibition of the rickettsial yield, suggesting that de novo protein synthesis is not required for
more » ... he inhibitory effect of rTNF-a1. The addition of tryptophan partially abolished the inhibitory effect of rTNF-a and rTNF-a plus gamma interferon.
doi:10.1128/iai.58.6.1886-1890.1990 fatcat:aezuin2vzbg33ducdzt3d65whq