Introduction. Atenolol -4-[2 -Hydroxy -3-[(1-methylethyl) amino] propoxy] benzene acetamide -β-adrenoceptor blocking agent is used to treat stenocardia, arterial hypertension, tachycardia, myocardial infarction [1, 2] . Atenolol can cause bradycardia, hypotension, depression, hallucinations, allergies and as a result of overdose and self-treatment, it can cause intoxication of the organism and the lethal effects [3, 4] . For therapeutic monitoring during the treatment of patients, for the study

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... of biological objects on atenolol as a result of intoxication and lethal consequences, the use of highly sensitive, rapid and effective methods of analysis is important. The most common chromatographic methods for analysis of atenolol are gasliquid chromatography (GLC) [3], high performance liquid chromatography (HPLC) [3, 5] , gas chromatography-mass spectrometry (GC-MS) [6] . The previously developed methods of HPLC analysis of atenolol are distinguished by the use of different chromatographic conditions, which are based on the individual properties of investigated substance. Аn important stage for the further research of medicinal substances and their mixtures in biological objects is the development of a unified HPLC method and the creation of databases by the parameters of identification and quantitative determination of analytes. Purpose of workthe development of analysis of atenolol by unified HPLC method. Materials and methods of research. Chromatographic analysis was carried out on a microcolumn liquid chromatograph "Milichrome A-02" (EkoNova, Closed Joint-Stock Company, Novosibirsk, Russia) according to the unified HPLC methodology developed by the author -Baram G.Y. using standardized HPLC conditions: reversed-phase variant with using of metallic column with non-polar absorbent Prontosil 120-5C 18 AQ, 5 μm; mobile phase in the mode of linear gradientfrom eluent А (5 % acetonitrile and 95% buffer solution -0,2 М solution of lithium perchlorate in 0,005 М solution perchloric acid) to eluent B (100% acetonitrile) as during 40 min. Regeneration of column has been conducted during 2 min with