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Adaptation and Serial Passage of Bovine Coronavirus in an Established Diploid Swine Testicular Cell Line and Subsequent Development of a Modified Live Vaccine
[chapter]
1998
Advances in Experimental Medicine and Biology
A virulent bovine coronavirus isolate (newborn calf diarrheal) was adapted and serially passaged in an established diploid swine testicular cell line (ST cells). The same cells have been used to produce modified live porcine rotavirus and coronavirus vaccines that are federally licensed and sold worldwide. Growth of the bovine coronavirus resulted in cytopathic effect characterized by cellular stranding and subsequent cell lysis. Virus yields were relatively high in the ST cells and active
doi:10.1007/978-1-4615-5331-1_91
fatcat:5bxvh7v7jrcbxe6eyck2pxtpye