The hypothalamus is the key site of central regulation of energy homeostasis, appetite, and reproduction. The long form leptin receptor (Ob-Rl) is localized within the hypothalamus along with several neuropeptides that are involved in regulation of the neuroendocrine axis. In the present study, developmental changes in gene expression of the Ob-Rl, preproorexin, proopiomelanocortin (POMC), corticotropin releasing factor (CRF), somatostatin, and GnRH in the hypothalamus was studied. Expression

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... Ob-Rl and neuropeptide mRNA was examined by semiquantitative reverse transcription-polymerase chain reaction in hypothalami collected from 106-day-old fetus (n ‫؍‬ 3) and 7-day-old (n ‫؍‬ 3), 3.5-mo-old (n ‫؍‬ 3), and 6-mo-old (n ‫؍‬ 2) gilts. In addition, leptin mRNA expression in the first three ages was examined in back fat. Leptin mRNA expression increased (P Ͻ 0.05) by 7 days postnatal, but Ob-Rl mRNA expression increased (P Ͻ 0.01) by 3.5 mo. Expression of preproorexin (P Ͻ 0.05), somatostatin, and GnRH (P Ͻ 0.01) mRNA peaked by 3.5 mo of age while POMC mRNA expression increased markedly (P Ͻ 0.01) by 6 mo of age. The CRF mRNA expression did not change across ages. These findings suggest a possible relationship among Ob-Rl and a number of hypothalamic and peripheral peptides in the development of the neuroendocrine axis. These peptides may serve as messengers that link mechanisms that regulate reproduction and energy balance. developmental biology, hypothalamic hormones, hypothalamus, leptin, leptin receptor, neuropeptides