The main objective of this study was to determine the prevalence and antimicrobial resistance profiles of the main bacteria which are responsible for urinary tract, blood stream, cerebrospinal fluid, lower respiratory tract, and wound infections in Hamadan province in the west of Iran. Materials and Methods: In this study, a total of 773 urinary tract, 273 blood stream, 13 cerebrospinal fluid, 408 respiratory tract, and 147 wound positive samples were collected from patients who referred to

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... t hospital from April 2013 to October 2014. Antibiotic susceptibility testing was performed by Modified Disk Diffusion Method (MDDM) against different classes of antibiotic. Results: The most common pathogens isolated from urine tract, blood stream, cerebrospinal fluid, lower respiratory tract, and wound infections were E. coli 425 (54.9%), S. aureus 68 (24.9%), Klebsiella spp. 3 (23%), P. aeruginosa 110 (26.9%), and S. aureus 30 (20.4%) respectively. The overall prevalence of resistance to the antimicrobial agents tested in various clinical specimens is discussed in this study. Conclusion: The high resistance rate was observed in our study to most used antibiotics. Therefore, setting up a comprehensive surveillance system is need to evaluate the distribution of organisms isolated and their drug resistance pattern over different period of time and place of Iran.