Injectable Bone Tissue Engineering Using Expanded Mesenchymal Stem Cells

Yoichi Yamada, Sayaka Nakamura, Kenji Ito, Eri Umemura, Kenji Hara, Tetsuro Nagasaka, Akihiro Abe, Shunsuke Baba, Yasushi Furuichi, Yuichi Izumi, Ophir D. Klein, Toshihiko Wakabayashi
2013 Stem Cells  
Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectable
more » ... red bone (TEB) composed of mesenchymal stem cells (MSCs) and platelet-rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out large-scale clinical studies in patients with long-term follow-up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months were significantly increased over the preoperation baseline (p < .001) and reached levels equivalent to that of native bone. No significant bone resorption occurred during longterm follow-up. Injectable TEB restored masticatory function in patients. This novel clinical approach represents an effective therapeutic utilization of bone tissue engineering.
doi:10.1002/stem.1300 pmid:23225744 pmcid:PMC4043220 fatcat:c6zoakfoyjdwrao7gk6l4r7hhe