Lipid droplet structural remodeling in adipose tissue upon caloric excess [article]

Weinan Zhou, Sarith R Bandara, Cecilia Leal, Sayeepriyadarshini Anakk
2021 bioRxiv   pre-print
Excess calories are stored as triacylglycerols (TAG) and cholesteryl esters (CE) in lipid droplets (LD), and during obesity, LD expansion occurs. X-ray scattering of adipose tissue uncovered that LDs comprise two TAG packing domains: a disordered core and a multilamellar shell. The number of TAG layers increases upon diet-induced obesity and is adipose depot-specific. Further, collagen was highly oriented in brown but randomly dispersed in white fat. We discovered that the body's surfactant,
more » ... e acids (BAs) stimulate remodeling of LD size. Deleting the BA receptor, Farnesoid X receptor (FXR) reduced a hydrophilic BA, beta muricholic acid (beta-MCA), and enlarged the adipocytes. BA composition is a critical determinant of overall hydrophobicity index and solubilization ability. Accordingly, we found that the obesogenic diet reduced a hydrophobic BA, chenodeoxycholic acid (CDCA). Taken together, these findings implicate that BAs, tissue niches, and diet influence LD structural remodeling.
doi:10.1101/2021.08.23.457409 fatcat:smsdzhq6lbdf5cb7iwk2a6sgqy