Toxins produced from bacteria constitute promising antitumor agents in treating different cancer types. Exotoxin A from Pseudomonas aeruginosa is the highly toxic virulence component that bind to specific cell receptors. This study aimed to purify Exotoxin A from clinically isolated Pseudomonas aeruginosa. A total of 150 bacterial isolates were taken from clinical samples (burn and wounds) and were screened on selective media and identified as P. aeruginosa using biochemical analysis and

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... ar test by PCR amplification technique utilizing specific primer 16srRNA. Exotoxin A produced from P. aeruginosa were screened and purified by two steps that include gel filtration and ion-exchange chromatography. In this study, 68 isolates were characterized as P. aeruginosa, furthermore, real-time PCR proved that these isolates revealed 100% specificity, sensitivity and had positive amplified bands with a size of 249bp. The concentration of Exotoxin A extracted from P. aeruginosa using Trypticase soy broth was 18 Mg/ml. The percent of Purification and recovery for Exotoxin A was 21.4 %, 40% respectively. Clinical Isolates of Pseudomonas aeruginosa have the potential to produce Exotoxin A that is responsible for pathogenicity.