A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL.
The file type is
Removing Very Fine Wrinkles from Half-Micron Epoxy Sections
Very fine wrinkles can occur at the tissue-resin interface when cutting half-micron thick sections of e poxy-em bedded mouse intestine. The following is a list of possible remedies for removing such wrinkles: • Adding glycerol to the water in the knife boat • Using sodium ethoxide (or methoxide) to etch the resin • Using chloroform, xylene or trichloroethylene furmes to stretch the sections • Floating sections on a drop of 10% acetone on a slide, then heating gently to evaporate the acetone •doi:10.1017/s1551929500052330 fatcat:uoq3xqqfb5elpnaptvrv5xnuw4