The coenzyme thiamine diphosphate displays a daily rhythm in the Arabidopsis nucleus

Zeenat B. Noordally, Celso Trichtinger, Ivan Dalvit, Manuel Hofmann, Céline Roux, Nicola Zamboni, Lucille Pourcel, Elisabet Gas-Pascual, Alexandra Gisler, Teresa B. Fitzpatrick
2020 Communications Biology  
In plants, metabolic homeostasis-the driving force of growth and development-is achieved through the dynamic behavior of a network of enzymes, many of which depend on coenzymes for activity. The circadian clock is established to influence coordination of supply and demand of metabolites. Metabolic oscillations independent of the circadian clock, particularly at the subcellular level is unexplored. Here, we reveal a metabolic rhythm of the essential coenzyme thiamine diphosphate (TDP) in the
more » ... te (TDP) in the Arabidopsis nucleus. We show there is temporal separation of the clock control of cellular biosynthesis and transport of TDP at the transcriptional level. Taking advantage of the sole reported riboswitch metabolite sensor in plants, we show that TDP oscillates in the nucleus. This oscillation is a function of a light-dark cycle and is independent of circadian clock control. The findings are important to understand plant fitness in terms of metabolite rhythms.
doi:10.1038/s42003-020-0927-z pmid:32372067 fatcat:whu3bxtqbvaqlmcxcyzvyxzpg4