Location of cis-acting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2

E M McCann, A M Lever
1997 Journal of Virology  
We used a series of deletion mutations in the untranslated leader region of human immunodeficiency virus type 2 (HIV-2) to seek cis-acting packaging signals. Sequences between the 5 major splice donor and the gag initiation codon, where such signals have been identified in HIV-1, appear to make a measurable but very minor contribution to genomic RNA packaging, and deletions here had little effect on viral replication in vitro. Immediately 5 to the splice donor, two regions were identified
more » ... re identified which, when deleted, caused a significant replication defect. The most proximal of these to the splice donor demonstrated a phenotype consistent with its being a major cis-acting packaging signal in HIV-2.
doi:10.1128/jvi.71.5.4133-4137.1997 fatcat:7yic4cc3krdgllzshhmxzmuusi