‫ﺑﻨﯿﺎدی‬ ‫ﻫﺎی‬ ‫ﺳﻠﻮل‬ ‫ﺷﻨﺎﺳﺎﯾﯽ‬ ‫و‬ ‫ﺟﺪاﺳﺎزی‬ ‫ﻗﺪرت‬ ‫ﺑﺮرﺳﯽ‬ ‫و‬ ‫اﻧﺴﺎن‬ ‫رﺣﻢ‬ ‫اﻧﺪوﻣﱰ‬ ‫ﺳﻠﻮﻟﯽ‬ ‫ﳐﺘﻠﻒ‬ ‫ﻫﺎی‬ ‫رده‬ ‫ﺑﻪ‬ ‫ﺎ‬‫آ‬ ‫ﲤﺎﯾﺰی‬ ‫ﲰﯿﻪ‬ ‫ﺑﺎروق‬ ‫اﺑﺮاﻫﯿﻤﯽ‬ Isolation and characterization of human endometrial stem cells and evaluation of their differentiation potential

S Ebrahimi-Barough, J Ai, H Kouchesfehani, Mohammad Nabiyuni
unpublished
Human endometrial stem cells (hEnSCs) contain a cell population that are capable of self-renewal and show similar properties to mesenchymal stem cells. We show that these cells can differentiate to different cell lineages. Materials and Methods: In this study, endometrial tissue obtained from patients and then cells were extracted anzymaticaly. The released cells were cultured in DMEM/F12 with 10% FBS. The flow cytometry analysis was done for CD105, CD90, CD146, CD31 and CD34 in the third
more » ... in the third passage. The adipogenic, osteogenic, neurogenic and endothelial differentiation was evaluated in the third passage after 21 days of induction with differentiation media. Results: The flow cytometry analysis showed that EnSCs were positive for CD90, CD105 and CD146 and were negative for CD31 and CD34. Moreover, we showed that the hEnSCs can differentiate to osteocyte and adipocyte that confirmed by alizarin Red and oil-Red O staining. The differentiated cells into neuron and endothelial expressed related markers including Nestin, MAP2 and CD31. Discussion: Results showed that hEnSCs appear to be able to provide a source of different kinds of cells that will be able to be used to further study the cellular differentiation processes and cell therapy.
fatcat:kauibwjukre5xmpfzcfatqeiia