Detection of Gardnerella vaginalis in vaginal specimens by direct immunofluorescence

W Hansen, B Vray, K Miller, F Crokaert, E Yourassowsky
1987 Journal of Clinical Microbiology  
The preparation of a fluorescein-labeled Gardnerella vaginalis polyclonal antibody is described, and its usefulness is assessed for the detection of this microorganism in vaginal samples obtained from 263 women attending the gynecological department of a general hospital, 66 of whom harbored an intrauterine device. The direct immunofluorescence technique was positive for G. vaginalis in 21 % of the specimens, whereas only 12.5% of the total bacteriological cultures were positive. The frequency
more » ... ive. The frequency was higher in patients harboring intrauterine devices since 34.8% exhibited positive immunofluorescence and 30.3% exhibited positive cultures.
doi:10.1128/jcm.25.10.1934-1937.1987 fatcat:mpyq2bu5tjbgvc7ea4zatvygh4