Dual spatial maps of transcript and protein abundance in the mouse brain

Christopher C Park, Vladislav A Petyuk, Wei-Jun Qian, Richard D Smith, Desmond J Smith
2009 Espert Review of Proteomics  
Integrating quantitative proteomic and transcriptomic datasets promises valuable insights in unraveling the molecular mechanisms of the brain. We concentrate on recent studies using mass spectrometry and microarray data to investigate transcript and protein abundance in normal and diseased neural tissues. Highlighted are dual spatial maps of these molecules obtained using voxelation of the mouse brain. We demonstrate that the relationship between transcript and protein levels displays a
more » ... anatomical distribution, with greatest fidelity in midline structures and the hypothalamus. Genes are also identified that have strong correlations between mRNA and protein abundance. In addition, transcriptomic and proteomic analysis of mouse models of Parkinson's disease are discussed. The mammalian brain is a complex organ exhibiting a rich variety of gene expression patterns across a broad range of cell types. In-depth multimodal study is required to understand this complex network of cells and their associated transcripts and proteins. Recent advances in the quantitative detection of mRNA and proteins on a genomic scale permit localization of these
doi:10.1586/epr.09.46 pmid:19489697 pmcid:PMC2744401 fatcat:ah6ckkw76remrdkoof7xbmi3im