Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification

Shiwei Ma, Xue Li, Bo Peng, Weihua Wu, Xin Wang, Hui Liu, Lihong Yuan, Shisong Fang, Jiahai Lu
2018 Biological and Pharmaceutical Bulletin  
Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA
more » ... were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time reverse transcription polymerase chain reaction (RT-PCR) assay. The results data demonstrated that our method performed well and could can be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9. Keywords Recombinase polymerase amplification · Lateral flow dipstick · Avian influenza A virus · H7N9 Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples. Am J Trop Med Hyg, 92, 583-587 (2015). 12) Tu PA, Shiu JS, Lee SH, Pang VF, Wang DC, Wang PH: Development of a recombinase polymerase amplification lateral flow dipstick (RPA-LFD) for the field diagnosis of caprine arthritis-encephalitis virus (CAEV) infection. J Virol Methods, 243, 98-104 (2017). 13) Yehia N, Arafa AS, Abd El Wahed A, El-Sanousi AA, Weidmann M, Shalaby MA: Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection.
doi:10.1248/bpb.b18-00468 pmid:30232304 fatcat:fvgpxkx2orf2vd7cjh3esaq3ra