Cathepsin B deficiency attenuates cardiac remodeling in response to pressure overload via TNF-α/ASK1/JNK pathway

Qing-Qing Wu, Man Xu, Yuan Yuan, Fang-Fang Li, Zheng Yang, Yuan Liu, Meng-Qiao Zhou, Zhou-Yan Bian, Wei Deng, Lu Gao, Hongliang Li, Qi-Zhu Tang
2015 American Journal of Physiology. Heart and Circulatory Physiology  
sin B (CTSB), a member of the lysosomal cathepsin family that is expressed in both murine and human hearts, was previously shown to participate in apoptosis, autophagy, and the progression of certain types of cancers. Recently, CTSB has been linked to myocardial infarction. Given that cathepsin L, another member of the lysosomal cathepsin family, ameliorates pathological cardiac hypertrophy, we hypothesized that CTSB plays a role in pressure overload-induced cardiac remodeling. Here we report
more » ... at CTSB was upregulated in cardiomyocytes in response to hypertrophic stimuli both in vivo and in vitro. Moreover, knockout of CTSB attenuated pressure overloadinduced cardiac hypertrophy, fibrosis, dysfunction, and apoptosis. Furthermore, the aortic banding-induced activation of TNF-␣, apoptosis signal-regulating kinase 1 (ASK1), c-Jun NH2-terminal kinases (JNK), c-Jun, and release of cytochrome c was blunted by CTSB deficiency, which was further confirmed in in vitro studies induced by angiotensin II. In cardiomyocytes pretreatment with SP600125, a JNK inhibitor, suppressed the cardiomyocytes hypertrophy by inhibiting the ASK1/JNK pathway. Altogether, these data indicate that the CTSB protein functions as a necessary modulator of hypertrophic response by regulating TNF-␣/ASK1/JNK signaling pathway involved in cardiac remodeling.
doi:10.1152/ajpheart.00601.2014 pmid:25713304 fatcat:wuoknqet3zhhrlg5p7l3gyrbga