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Optimization of a Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells
2018
Acta Medica Marisiensis
Objective: Peripheral blood mononuclear cells (PBMC) are extremely important in the body's immune response. Their isolation represents a major step in many immunological experiments. In this two phase study, we aimed to establish an optimum protocol for PBMC isolation by density-gradient centrifugation. Methods: During Phase-1, we compared two commercially available PBMC isolation protocols, Stemcell Technologies (ST) and Miltenyi Biotec (MB), in terms of PBMC recovery and purity. Twelve blood
doi:10.2478/amma-2018-0011
fatcat:rhu3tnt46fc3peig4mmlnx2qxa