Effects of the ionic imbalance in the fertigation solution on growth, proline content and nitrate reductase activity in muskmelon seedlings
J. Olave, Centro de Investigación y Desarrollo en Recursos Hídricos -CIDERH, Iquique, Chile, M. Guzman, A. Sanchez, C. Santander, University Arturo Prat, Iquique, Chile, Cei A3 -CIEMBITAL.Almería University, Almería, Spain, Cei A3 -CIEMBITAL.Almería University, Almería, Spain, Centro de Investigación y Desarrollo en Recursos Hídricos -CIDERH, Iquique, Chile, University Arturo Prat, Iquique, Chile
2018
Net Journal of Agricultural Science
The aim of this research was to evaluate the effects of ionic imbalance of fertigation solution in muskmelon (Cucumis melo) seedlings on growth, proline content and nitrate reductase activity (NRA). 'Galia' melon seeds were primed with 8 g·L -1 of N different N-form fertilizers (NH 4 NO 3 , KNO 3 or NH 4 NO 3 +KNO 3 ), or nonprimed (Control). These seeds were germinated and grown in perlite inside a passive greenhouse nursery. The water quality of "place Pintados region Tarapacá -Chile" was
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... oduced (EC: 2.6 dS m -1 , pH: 7.3; Na + : 12.2 meq L -1 , Cl -: 18.12 meq L-1, SAR adj : 10.4). Four fertigation solutions were prepared with this water, maintaining a K + /(Ca 2+ + Mg 2+ ) ratio of 0.4 and pH between 6.1 and 6.6, but modifying EC (3.0; 4.1; 5.1; 6.2), SAR adj (10.4; 9.2; 8.1; 7.0) and NO 3 -/Clratio (0.47;0.73;0.86;1.25). The ionic ratio in fertigation solutions was modified during germination and seedling growth, which led to an increase of NO 3 from 8.6 to 22.8 meq L -1 . Seedlings fertigated with 5.1 dS m -1 (SAR adj : 8.1) nutrient solution showed the highest plant height, leaf number and leaf area, but there was no significant effect on fresh and dry biomass. Proline content and NRA showed a high correlation with NO 3 concentration. The lowest proline content was obtained at 12 meq L -1 NO 3 in the fertigation solution, indicating the lowest stress level and the highest efficiency in nitrogen absorption. Moreover, proline levels decreased before seedlings were planted. This different behaviour between endogenous and induced NRA may be useful to optimize NO 3 supply and management in the nursery. REFERENCES Akinci I, Simsek M, 2004. Ameliorative effects of potassium and calcium on the salinity stress in embryo culture of cucumber (Cucumis sativus L.). J Biol Sci, 4: 361-365. Bates LS, Waldren RP, Teare ID, 1973. Rapid determination of free proline in water stress studies. Plant Soil, 39: 205-208. Clauseen W, 2002. Growth, water use efficiency, and proline content of hydroponically grown tomato plants as affected by nitrogen source and nutrient concentration. Plant Soil, 247: 199-209. Cuartero J, Fernández-Muñoz R, 1999. Tomato and salinity. Sci Hort, 78: 83-125. Filgueira R, Sarli G, Jatimliansky J, 1998. Relations between specific leaf surface and gas exchange in triticum genotypes. Rev Fac Agron, 103: 177-184. Flores P, Botella M, Martínez V, Cerda A, 2000. Ionic and osmotic effects on nitrate reductase activity in tomato seedlings. J Plant Physiol, 156: 552-557. Franco J, Esteban C, Rodríguez C, 1993. Effects of salinity on various growth stages of muskmelon cv. Regival. J Hort Sci, 68: 899-904. Franco J, Fernández J, Bañon S, 1997. Relationship between the effects of salinity on seedling leaf area and fruit yield of six muskmelon cultivars. Hort Sci, 32: 642-644. Heder B, 1999. Osmoregulatory role of proline in plants exposed to environmental Stresses. p. 675-695. In: Pessarakli, M. (ed.), Handbook of Plant and Crop Stress, Second Edition, Revised and Expanded, Marcel Dekker, Innc., New York, U.S.A. Helal M, Koch K, Mengel K, 1975. Effect of salinity and potassium on the uptake of nitrogen and nitrogen metabolism in young barley plants. Physiol Plant, 35: 310-313. Jiang Z, Hull R, 1998. Interrelationships of nitrate uptake, nitrate reductase, and nitrogen use efficiency in selected Kentucky bluegrass cultivars. Crop Sci, 38: 1623-1632. Kaya C, Kirnak H, Higgs D, Saltali K, 2002. Supplementary calcium enhances plant growth and fruit yield in strawberry cultivars grown at high (NaCl) salinity. Sci Hort, 93: 65-74. Kubala S, Wojtyla L, Quinetc M, Lechowska K, Luttsc S, Garnczarska M, 2015. Enhanced expression of the proline synthesis gene P5CSA in relation to seed osmopriming improvement of Brassica napus germination under salinity stress. J Plant Physiol, 183: 1-12. Olave et al. 69 Citation: Olave J, Guzman M, Sanchez A, Santander Ch., 2018. Effects of the ionic imbalance in the fertigation solution on growth, proline content and nitrate reductase activity in muskmelon seedlings. Net J Agric Sci, 6(4): 63-69.
doi:10.30918/njas.64.18.031
fatcat:qmvvxizwkzarpfmftawzn5tsvq
