& objectives: There is no overall estimation for prevalence of surface antigen of hepatitis B (HBsAg) in Iranian health care workers; though various studies conducted on health care settings reported different rates. Present study aimed to evaluate the prevalence of HBsAg in Iranian health care workers using systematic review and Meta analysis method. Methods: This Meta analysis study was performed using PRISMA checklist. Iranmedex, SID, Medlib, Scopus, Pubmed, Science Direct, Cochrane, Embase,

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... Medline, Springer, Wiley Online Library, and Google Scholar databases were searched to access to the papers published till 2015 in Persian and English languages using standard key words. HBsAg+ was the study criterion. Searching and data extraction were done by two independent reviewers. Random effects model in meta-analysis was used to pool the data. Results: 17 studies with 3002 subjects were included in the study. The prevalence of HBsAg+ was estimated to be 0.4 %(95% CI: 0.1-0.6) among Iranian health care workers. The respective minimum and maximum rates were observed in central (0.3%) and west (4.1%) of the country. Conclusion: The prevalence of HBsAg+ in Iranian health care workers was unexpectedly lower than the general population. It seems that preventive measures were better applied for this occupation than the general population to reduce prevalence of hepatitis B infection which has not been significantly increased in the last 26 years.