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Microinjected Fluorescent Phalloidin in vivo Reveals the F-Actin Dynamics and Assembly in Higher Plant Mitotic Cells
The Plant Cell
Endosperm mitotic cells microinjected with fluorescent phalloidin enabled us to follow the in vivo dynamics of the F-actin cytoskeleton. The fluorescent probe immediately bound to plant microfilaments. First, we investigated the active rearrangement of F-actin during chromosome migration, which appeared to be slowed down in the presence of phalloidin. These findings were compared with the actin patterns observed in mitotic cells fixed at different stages. Our second aim was to determine thedoi:10.2307/3868925 pmid:2136631 fatcat:yqmkj7r5szgfti3xgjemlo6ikm