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Simultaneous Visualization of Myosin Heavy Chain Isoforms in Single Muscle Sections
2013
Cells Tissues Organs
We developed a staining protocol that enables simultaneous visualization of myosin heavy chain (MHC) pure and hybrid muscle fiber types in rat skeletal muscle. Up to eight different muscle fiber types can be visualized in a single section of the rat extensor digitorum longus muscle, which contains all four adult MHC isoforms and shows plasticity during the dennervation-reinnervation process. Triple immunofluorescent staining of MHC-1, MHC-2a and MHC-2b with primary antibodies BA-D5 (isotype
doi:10.1159/000345424
pmid:23306777
fatcat:w5oyjd7jize4hhbizfjuxavizm