Hospital infections caused by fungi have been frequently reported in hospitalized patients, with a high morbidity and mortality, making it increasingly important to the awareness of air quality. This study is designed to isolate, identify and investigate the occurrence of airborne fungal spores present in various working environments of some selected hospitals within Lagos State, Nigeria. Air samples at six working area (reception, out-patient, male ward, female ward, maternity ward and

more »

... g theatre) of the selected hospitals were obtained on triplicate of Sabouraud dextrose agar plates, every week days for six consecutive weeks. Isolated fungi were subsequently identified using both microscopic and macroscopic characteristics. Seven species of fungi belonging to three genera were recovered during this study period; Aspergillus niger, A. flavus, A. fumigatus, A. species, Candida species, Penicillium citrinum and P. species. The most abundant fungus encountered in this study is A. niger with P. species being the least isolated fungus from all the investigated hospitals. Of the six working areas sampled in the eight hospitals, the reception and out-patient areas appeared to record the highest occurrence of encountered fungi while the examined operating theatre rooms had the least fungal airsporal contaminant. It was gathered from this study that despite the daily cleaning of the investigated hospital working environments with different antiseptic and disinfectants, fungal contaminant was observed throughout the sampled period. We thereby conclude that the hospital environments are continuously monitored in order to identify and eliminate the sources of these fungal spores.