Size uniformity of animal cells is actively maintained by a p38 MAPK-dependent regulation of G1-length [article]

Shixuan Liu, Miriam Bracha Ginzberg, Nish Patel, Marc Hild, Bosco Leung, Yen-Chi Chen, Zhengda Li, Nancy Chang, Shulamit Diena, Yuan Wang, William Trimble, Larry Wasserman (+3 others)
2017 bioRxiv   pre-print
Animal cells within a tissue typically display a striking regularity in their size. To date, the molecular mechanisms that control this uniformity are still unknown. We have previously shown that size uniformity in animal cells is promoted, in part, by size-dependent regulation of G1 length. To identify the molecular mechanisms underlying this process, we performed a large-scale small molecule screen and found that the p38 MAPK pathway is involved in coordinating cell size and cell cycle
more » ... sion. Small cells display higher p38 activity and spend more time in G1 than larger cells. Inhibition of p38 MAPK leads to loss of the compensatory G1 length extension in small cells, resulting in faster proliferation, smaller cell size and increased size heterogeneity. We propose a model wherein the p38 pathway responds to changes in cell size and regulates G1 exit accordingly, to increase cell size uniformity.
doi:10.1101/119867 fatcat:ivfcbp5an5h2fljag3nlflnvue