Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12

J C Lazzaroni, R C Portalier
1981 Journal of Bacteriology  
Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and
more » ... icillin. Furthermore, they were resistant to colicin El and partially resistant to phage TuIa. Their genetic characterization showed that the Iky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.
doi:10.1128/jb.145.3.1351-1358.1981 fatcat:4l46xlacdvbwndv4xkbjlglupy