Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma

Xudong He, GuangYi Yang, E. Yang, Moli Zhang, Dan Luo, Jingjian Liu, Chongnan Zhao, Qinhua Chen, Fengying Ran
2021 Sensor Review  
Purpose Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed. Design/methodology/approach Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon
more » ... ng PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence. Findings This sensor can detect PD-L1 with a linear range from 100 pg mL−1 to 100 ng mL−1, and a detection limit of 10 pg•m−1 was achieved. Originality/value This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.
doi:10.1108/sr-04-2020-0087 fatcat:crz3uzqkobehjg4egto252uvtm