Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: mechanism of the phosphoryl-group transfer from phosphoenolpyruvate to HPr

Onno Misset, George T. Robillard
1982 Biochemistry  
The mechanism of phosphoryl-group transfer from phosphoenolpyruvate (PEP) to HPr, catalyzed by enzyme I of the Escherichia coli PEP-dependent phosphotransferase system, has been studied in vitro. Steady-state kinetics and isotope exchange measurements revealed that this reaction cannot be described by a classical ping-pong mechanism although phosphoenzyme I acts as an intermediate. The kinetic
doi:10.1021/bi00256a016 pmid:7049237 fatcat:dxplhowsdzbyfhdazgg6ej7fdi