Efeitos da hipercolesterolemia sobre os epitélios da mucosa bucal e capacidade de cicatrização em idades precoces - crianças e jovens (estudo experimental) [thesis]

Gilberto André e Silva
Obesity in childhood has becoming a concern. Obesityy is associated with increased triglycerides, decreased high-density lipoprotein cholesterol levels, and increased low-density lipoprotein cholesterol and may lead to increased risk for hypercholesterolemia and cardiovascular complications in adult life. Clinical symptoms and signs of elevated cholesterol levels do not occur until adulthood, but subclinical aspects may be observed in children and adoslecentes. There are no studies on the
more » ... tudies on the effects of hypercholesterolemia in children, adolescents or in adults on oral tissues. We propose to study the influence of early age hypercolesterolemia in epithelial oral tissues and on wound healing. Male albino Wistar rats (40g) received enriched cholesterol diet (1%) for 2 and six weeks for epithelial evaluation. After 2 weeks, one control group (receiving standard pellet diet) and treated (hypercholesterolemic diet) received surgical wounds at the dorsum. Animals were sacrifed 1, 3 and 7 days after surgery. At the end of both periods (2 and 6 weeks) animals were sacrificed for epithelial evaluation. At the sacrifice time, serum were collected for evaluation of triglycerides, total cholesterol, HDL, LDL and VLDL. Were collected skin, tongue, palate and gingival tissues. Skin samples were also used for evaluation of the wound healing. All the observation were made at light microscopy. Data were statistically analysed by Mann Whitney U test for comparison between two samples. P[U] ≤0.05 were considered statistically significant. The enriched cholesterol diet significantly increased the total cholesterol, triglycerides, LDL and VLDL plasma content, and the study provide support for the hypothesis that hypercholesterolemia may be associated with deficiencies in the wound healing process. An important clinical implication of this study is the identification of alterations epithelium on oral tissues and in early phases of wound healing associated with subclinical but important alterations in cholesterol levels.
doi:10.11606/d.58.2009.tde-26032010-154120 fatcat:jerlcx6sdvc3vbnkreo6vawe6y