Effect of triptolide on the regulation of ATP-binding cassette transporter A1 expression in lipopolysaccharide-induced acute lung injury of rats

JUN CHEN, JIANLIN GAO, JIANPING YANG, YUKUN ZHANG, LINA WANG
2014 Molecular Medicine Reports  
The aim of this study was to investigate the effect of triptolide on ATP-binding cassette transporter A1 (ABCA1) expression in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. Thirty male Sprague Dawley rats weighing 200-250 g were randomly divided into six groups: Normal (N, n=5), Control (C, n=5), LPS (L, n=5), Triptolide 25 µg (TP 1 , n=5), Triptolide 50 µg (TP 2 , n=5) and Triptolide 100 µg (TP 3 , n=5). The N group was not administered anything; the C group was
more » ... oup was administered 5 ml/kg normal saline intravenously and 7.5 ml/kg 1% dimethylsulfoxide (DMSO) intraperitoneally; the L group was administered 5 mg/kg 0.1% LPS and 1% DMSO; and the TP 1 , TP 2 and TP 3 groups were separately injected with 0.1% LPS and 25, 50 or 100 µg/kg triptolide, respectively. All groups had the same liquid-injection volume. Arterial blood gases, tumor necrosis factor-α (TNF-α) and ABCA1 expression and general pathology were examined following the treatments. It was found that increasing the triptolide dose in the TP 1-3 groups resulted in an increase in the expression of ABCA1 mRNA and protein. As compared with the L group, the ABCA1 expression showed a significant increase in TP 2 and TP 3 groups (P<0.05). In addition, the expression level of TNF-α was significantly increased in the L and TP 1 groups, as compared with that in the N or C groups (P<0.05). Conversely, a marked decrease in TNF-α expression was detected in the TP 2 and TP 3 groups, as compared with the L or TP 1 groups (P<0.05). In conclusion, this study found that triptolide could promote the expression of ABCA1 mRNA and protein and inhibit other inflammatory factors during LPS-induced ALI in rats. Regulating the expression of ABCA1 may be one of the protective mechanisms of triptolide. Furthermore, triptolide-induced increases in ABCA1 expression occurred in a dose-dependent manner between 25 and 100 µg/kg.
doi:10.3892/mmr.2014.2636 pmid:25323823 fatcat:n4fq4im22bb6zbper2pu2qsf3u