Development of Modified Kato Katz Kit as Laboratory Scale Prototype for Early Detection of Soil-Transmitted Helminths (STHs) Infection

Rifqoh Rifqoh, Yayuk Kustiningsih, Jujuk Anton Cahyono
2022 Medical Laboratory Technology Journal  
<span lang="EN-US">Early detection of </span><span lang="IN">Soil-Transmitted Helminths (STHs)</span><span lang="IN"> infection</span><span lang="EN-US"> in laboratory </span><span lang="IN">medical facilities </span><span lang="EN-US">is one of the government's efforts in </span><span lang="IN">its elimination</span><span lang="EN-US">. Government regulations recommend Kato</span><span lang="IN">-</span><span lang="EN-US">Katz's </span><span lang="IN">quantitative </span><span
more » ... od </span><span lang="IN">for</span><span lang="IN">the diagnosis </span><span lang="EN-US">of </span><span lang="IN">STHs infection</span><span lang="EN-US">. Kato</span><span lang="IN">-</span><span lang="EN-US">Katz's domesti</span><span lang="IN">c product</span><span lang="EN-US"> is not available</span><span lang="EN-US">yet</span><span lang="IN">.</span><span lang="EN-US"> Th</span><span lang="IN">is</span><span lang="EN-US"> research aims to create a laboratory-scale</span><span lang="IN"> modified</span><span lang="EN-US"> Kato</span><span lang="IN">-</span><span lang="EN-US">Katz kit for early detection of</span><span lang="IN"> STHs</span><span lang="IN">infection by fecal egg counts (FECs) in estimating the number of eggs per gram of stool (EPG).</span><span lang="IN">An experimental study was conducted to create a resin template, spatulas, and 3M-adhesive tape as a modified Kato-Katz Kit. 120 </span><span lang="EN-US">Kato-Katz thick smear preparations were done</span><span lang="IN"> from 30 samples for modified and standard Kato-Katz with duplication. The</span><span lang="EN-US"> amount of stool for each</span><span lang="IN"> was weighed. All Kato-Katz thick smear microscopic examination was done at least 60 minutes by identifying the STHs eggs and counting them.</span><span lang="EN-US">The result</span><span lang="IN"> for both methods</span><span lang="EN-US"> showed</span><span lang="IN"> no differences in EPG for Trichuris trichiura eggs p-value=0.012 (p&lt;0.05), meanwhile the differences for Ascaris lumbricoides eggs p-value=0.156, and Hookworm eggs p-value=0.102 (p&gt;0.05). Under microscopic examination, some A.lumbricoides eggs morphology was not visible because the adhesive in modified Kato-Katz blurry it, and the Hookworm fragile eggshells made it disappear within less than 60 minutes.</span><span lang="EN-US">The study concluded</span><span lang="IN"> no difference in EPG for T.trichiura eggs by FECs between</span><span lang="IN">modified</span><span lang="EN-US"> Kato-Katz</span><span lang="IN">as this study product with the standard. It is recommended to improve modified Kato-Katz in further study, especially the adhesive tape, before using it as a quantitative tool</span><span lang="IN">for</span><span lang="IN">early </span><span lang="EN-US">detection of </span><span lang="IN">STHs infection.</span><p> </p>
doi:10.31964/mltj.v7i2.419 fatcat:6rlxrfjwhjhxrptocai622b5ty