pH-Dependent Quenching of the Fluorescence of Tryptophan Residues in Class A β-Lactamase from E. coli (TEM-1)

Christo Christov, Dimitar Ianev, Alexandra Shosheva, Boris Atanasov
2004 Zeitschrift für Naturforschung C - A Journal of Biosciences  
We performed an investigation of the pH-dependent quenching of the fluorescence of tryptophan residues of TEM-1 β-lactamase from E. coli by uncharged and charged quenchers. pH-dependent Stern-Volmer constants (KSV/pH) of tryptophan residues allowed us to determine subtle but discrete structurally and functionally important processes.
doi:10.1515/znc-2004-11-1210 fatcat:nqncgnkokbewhdo2qva2txiw5e