Apolipoprotein A-V association with intracellular lipid droplets

Xiao Shu, Joanne Chan, Robert O. Ryan, Trudy M. Forte
2007 Journal of Lipid Research  
Apolipoprotein A-V (apoA-V) plays a key role in the regulation of triglyceride (TG) metabolism. Given the very low concentration of apoA-V in plasma, we hypothesized that apoA-V may influence plasma TG levels by affecting the assembly and/or secretion of apoB-containing lipoproteins. When apoA-V was overexpressed in cultured Hep3B cells, neither the amount of apoB secreted nor the density distribution of apoB-containing lipoproteins was affected. Fluorescence microscopy and cell lysate
more » ... cipitation studies revealed that apoA-V is not associated with apoB intracellularly, yet immunoprecipitation of apoA-V from the cell culture medium resulted in coprecipitation of apoB. These data suggest that the apoA-V association with apoB-containing lipoproteins is a postsecretory event. Confocal fluorescence microscopy revealed the presence of apoA-V in distinct cellular structures. Based on Nile Red staining, we identified these structures to be intracellular lipid droplets. These data suggest that apoA-V has a unique association with cellular lipids and, therefore, may be involved in the storage or mobilization of intracellular lipids.-Shu, X., J. Chan, R. O. Ryan, and T. M. Forte. Apolipoprotein A-V association with intracellular lipid droplets. Abbreviations: apoA-V, apolipoprotein A-V; GFP, green fluorescent protein; Met/Cys, methionine/cysteine; OA, oleic acid; TG, triglyceride.
doi:10.1194/jlr.c700002-jlr200 pmid:17460328 fatcat:lchbjsif3ngixl5sfclaok54xy