Diffuse Large B-Cell Lymphoma Initially Manifesting in the Bone Marrow

Dai Kajiura, Yoriko Yamashita, Naoyoshi Mori
2007 American Journal of Clinical Pathology  
Hematopathology / ORIGINAL ARTICLE CD34 (Novocastra Laboratories, Newcastle upon Tyne, England); and CD5 (T cell Diagnostics, Cambridge, MA). We evaluated the degree of neoplastic cell infiltration in sinusoids of the bone marrow in 4 grades using factor VIII staining: grades 0, 1, 2, and 3 represent 0%, 0% to 10%, 10% to 20%, and more than 20% of the total neoplastic cells, respectively. We also examined hemophagocytosis using H&E-stained specimens of the bone marrow and CD68 staining, which
more » ... 8 staining, which is a marker of histiocytes. Mild, moderate, and severe degrees represent 1 to 3 cells, 4 to 6 cells, and more than 7 cells in 10 high-power fields, respectively.
doi:10.1309/2gw5w7kqbxf6lfaw pmid:17439835 fatcat:6bpwmniuozc7ro253s7vefswzm