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Our results demonstrate that ethanol treatment can be used to evaluate large collections of environmental samples for the presence of giant viruses and to provide insight into understanding their ecology. This study should also facilitate the isolation of giant viruses using other species of protozoa in addition to Acanthamoeba spp. Abstract Objective: Giant viruses of the Megavirales order have been neglected in the literature because they are removed from samples during viral purification fordoi:10.1159/000354566 pmid:24157890 fatcat:kmqwfgsmcfgahfxaccicnxoznu