BGRS/SB-2022 799 A new approach to stimulating the wound healing based on Opisthorchis felineus proteins

2022 The Thirteenth International Multiconference   unpublished
Motivation and Aim: Skin regeneration is a natural homeostatic process, however, in some cases, chronic non-healing wounds or abnormal scarring develop. This situation is typical for such pathological conditions as deep mechanical damage, severe burns, old age, obesity, diseases of the circulatory system, as well as diabetes mellitus and other autoimmune diseases [1] . Currently, the correction of non-healing wounds is extremely difficult, which dictates the search for new promising therapeutic
more » ... agents. It should be noted the difficult task of finding drugs that can non-specifically stimulate the processes of skin regeneration, regardless of endo-and/or exogenous triggers of pathological nonhealing of wounds. In this regard, the search for new non-specific stimulators of skin regeneration of plant and animal origin seems to be highly relevant. The trematode Opisthorchis felineus infects the hepatobiliary system of fish-eating mammals [2] . These parasites have the ability to reduce acute inflammation, and also cause not only damage, but also stimulate the proliferation of bile duct cholangiocytes [3, 4] . Based on this, the aim of the study is to investigate the properties of O. felineus proteins as a wound healing agent. Methods and Algorithms: 80 male mice of the C57Bl/6 line were inflicted with superficial wounds with a diameter of 8 mm. Further, the animals were divided into 8 groups: control (chlorhexidine, 1.5 % methylcellulose, BSA) and experimental (ESP 1, 10, 10 μg without endotoxin; lysate 10 and 50 μg). After applying the solutions, the wound was treated with Luxplast spray plaster (Farmac-zabban, Italy), which created a water-repellent film to protect against contamination and fix the solutions. All groups will receive treatment every 3 days of the experiment with simultaneous detection of the wound area. Animals will be withdrawn from the experiment on days 7 and 10 of treatment. Damaged skin samples were taken for histological examination (formalin and freezing) and for the evaluation of gene expression (RNA-later). Results: ESP and O. felineus lysate proteins significantly increased wound healing in mice (p < 0.05). Histological methods showed that accelerated overgrowth was accompanied by a significant decrease in the area of inflammatory infiltration. On 10 th day of the experiment, there was no wet crust in the treatment groups, and reepetelization was detected in the lysate groups. In addition, by day 10, an increase in the number of newly formed CD34+ vessels was diagnosed in the treatment groups, which indicates an improvement in tissue trophism. According to the results of analysis of the expression of genes-markers of inflammation (Bl_4, Nos2, Arg1), organization of the extracellular matrix (Acta2, Col1α, Col 3,
doi:10.18699/sbb-2022-462 fatcat:kiooftmilncobhojl27okewije