Resuspend Label-IT amine at 1 mg/mL using the provided resuspension buffer. Add the resuspension buffer and vortex to mix. Resuspend acryloyl X SE (AcX) (https://www.lifetechnologies.com/order/catalog/product/A20770) in 500 µL anhydrous DMSO (10 mg/mL stock solution). Aliquot in 20 µL and store desiccated at -20°C. React the Label-IT to AcX at equal mass ratio. For example, add 1 µL of acryloyl X to 10 µL of Label-IT (both are resuspended in DMSO so the reaction is carried out in DMSO). React

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... r >6 hrs at room temperature on benchtop shaker. Store the LabelX at -20°C with desiccant for future use.