Comparação entre microdiluição e disco difusão para o teste de susceptibilidade aos antifúngicos contra Candida spp

Antônio Alexandre de Vasconcelos Júnior, Everardo Albuquerque Menezes, Francisco Afrânio Cunha, Maria da Conceição S Oliveira Cunha, Bárbara Helena Lima Braz, Ligya Guimarães Capelo, Carlla Lorena Façanha Silva
2012 Semina : Ciências Biológicas e da Saúde  
The evolution of fungal resistance can be evaluated by testing susceptibility to antifungal agents. The protocols for susceptibility testing most common are Broth Microdilution (BMD), Disk Diffusion (DD) and E-test. The objective of this study was to compare the susceptibility tests conducted by the BMD and DD methods for amphotericin B and fluconazole against Candida spp. isolated in Fortaleza/ CE. Were used 40 C. albicans, 50 C. tropicalis and 47 C. parapsilosis isolated from blood and urine
more » ... om blood and urine samples of patients treated at Fortaleza General Hospital. The tests were conducted by the BMD and DD methods according to protocols M27-A3 and M44-A2 of the CLSI. These methods were compared and the percentages of agreement were calculated. For amphotericin B and fluconazole was a predominance of strains with reduced susceptibility. The percentage of agreement for the methods tested was above 97%. No serious errors were detected. The use of DD for performing antifungal susceptibility testing can be applied in routine laboratory tests, since one technique is easy, inexpensive, and reliable when compared to the BMD without compromising the results for the strains of C. albicans, C. tropicalis and C. parapsilosis.
doi:10.5433/1679-0367.2012v33n1p135 fatcat:svu6t5bkonbm7goik5eo4uwbxu