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Verifying the Function and Localization of Genetically Encoded Ca2+Sensors and Converting FRET Ratios to Ca2+Concentrations
2015
Cold Spring Harbor Protocols
Genetically-encoded, ratiometric, fluorescent, Ca 2+ biosensors can be used in living cells to quantitatively measure free Ca 2+ concentrations in the cytosol or in organelles. This protocol describes how to perform a calibration of a Ca 2+ sensor expressed in cultured mammalian cells as images are acquired using a widefield fluorescence microscope. This protocol also explains how to calculate Förster Resonance Energy Transfer (FRET) ratios from acquired images and how to convert FRET ratios to
doi:10.1101/pdb.prot076547
pmid:25561614
pmcid:PMC4968932
fatcat:z66d7fvpqjecnlhrtrak73zna4