Metformin prevented high glucose-induced endothelial reactive oxygen species via OGG1 in an AMPKα-Lin-28 dependent pathway [article]

Liangliang Tao, Xiucai Fan, Jing Sun, Zhang Zhu
2020 bioRxiv   pre-print
Metformin improved vascular function in obese type 2 diabetic patients. 8-oxoguanine glycosylase (OGG1), a main DNA glycosylase, was necessary for vascular complications in diverse diseases. However, whether metformin suppressed endothelial ROS via OGG1 was unclear. Human umbilical vein endothelial cells (HUVECs) were exposed to metformin or HG (high glucose) for 0, 6, 12 and 24 hours. OGG1 and AMPKα expression was measured after metformin treatment while HG-caused ROS was measured by DHE
more » ... . Diabetic mice were induced by daily intraperitoneal injections of streptozotocin (STZ). Metformin significantly reduced endothelial ROS production via AMPKα-upregulating expression of OGG1, which was reversed by silence of Lin-28. These results suggested that metformin contacted HG-induced endothelial ROS via AMPKα/Lin-28/OGG1 pathway.
doi:10.1101/2020.11.18.388462 fatcat:ti2nrjfdzjenthg2nsdga73gh4