EXPRESSION OF PROGESTERONE-BINDING PROTEIN IN NORMAL AND NEOPLASTIC HUMAN ADRENALS
ヒト正常副腎および副腎腫瘍組織における progesterone 結合蛋白の発現

Takayoshi Demura, Yoshihiko Watarai, Masaki Togashi, Nobuo Ohashi, Tatsuya Chikaraishi, Tetsuo Hirano, Katsuya Nonomura, Tomohiko Koyanagi
1993 The Japanese Journal of Urology  
Specific progesterone-binding protein (P4-BP) is demonstrated in adrenocortical nuclei of the guinea pig, but, not in nuclei of other animals. We tried to demonstrate the progesterone-binding activity in nuclei of human normal adrenals and adrenal tumors. Normal adrenals were obtained from six patients with renal cell carcinomas undergoing radical nephrectomy. Seven adrenocortical adenomas were obtained: five tumors from patients with Cushing's syndrome, one tumor from nonfunctioning adenoma,
more » ... d one from aldosteronoma. Nuclei were purified from the tissues, and progesterone binding assay was performed. We could not demonstrated progesterone-binding activity in nuclei of six normal human adrenals. However, we demonstrated progesterone-binding activity in nuclei purified from human adrenocortical adenomas associated with Cushing's syndrome. Saturation analysis revealed a Kd of 13. 85 + 1. 99 nM (mean + SD, n=5) and a binding capacity of 1. 95 f 0. 37 pmol/mg DNA (mean f SD, n=5). A Kd of progestrerone-binding activity in human adrenocortical adenoma was similar to that of guinea pig P4-BP, and a binding capacity was about one-fifteenth of guinea pig P4-BP. However, nuclei purified from a non-functioning adrenocortical adenoma and an aldosteronoma failed to demonstrate progesterone-binding activity. The binding activity was specific for progesterone. 5a-pregnane-3, 20dione was a modest competitor, while, 17/3-estradiol, testosterone, cortisol, and other related steroids were poor competitors. Thus the progesterone-binding activity in human adrenals was similar to guinea pig P4-BP in the affinity and specificity of binding. Furthermore, we demonstrated [3H] progesterone bound to nuclei from normal human adrenals, as well as, from adrenocortical adenomas associated with Cushing's syndrome by means of thin layer chromatography. It is possible that the progesterone-binding activity is too low to be detected by the classical steroid binding assay.
doi:10.5980/jpnjurol1989.84.1286 pmid:8355444 fatcat:qqradmvyzjfjhec6ppe4um2iz4