Evidence that type III iodothyronine deiodinase in rat astrocyte is a selenoprotein

M. Ramauge
1996 Endocrinology  
A type III iodothyronine deiodinase (D-III) that inactivates thyroid hormones has been recently cloned and identified as a selenoprotein in neonatal rat skin. However, selenium (Se) deficiency does not affect the D-III activity in the rat placenta and decreases the D-III in the rat brain only slightly. This study examines the effect of Se on the D-III activity in cultures of rat brain astrocytes. Astrocytes were depleted in Se by maintaining them in Se-free chemically defined medium for 7 days.
more » ... medium for 7 days. These conditions decreased the activity of a recognized selenoprotein, glutathione peroxidase, 3-lo-fold. D-III activity induced by 12-0-tetradecanoylphorbol-13-acetate (TPA) was also
doi:10.1210/en.137.7.3021 pmid:8770927 fatcat:vlixlyvsyvcpnmm727kstb7tma