Background Plant cytochrome P450s are important in the biosynthesis of essential physiological compounds. However, the gene family of P450s in london planetree have not yet been reported.Objective To explore the sequence characteristics and expression pattern of P450s in london planetree.Methods The phylogenetic studies, evolutionary analysis,gene expression profiling, Weighted correlation network analysis of P450 genes in london planetree were conducted. The jasmonic acid pathway key

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... gene AOS was analyzed by qRT-PCR, cloned, homology modeled, and molecular docking analysis.Results The london planetree P450 genes were divided into 8 clans, 38 families, and 50 subfamilies. Among them, 48 genes showed a strong negative selec-tion of P450 genes between london planetree and Arabidopsis thaliana. Nine P450 genes were sig-nificantly up-regulated at 24 h and 48 h after feeding induction by sycamore lace bug Corythucha ciliata. After Weighted correlation network analysis (WGCNA), the P450 genes were identified by a co-expression network mod-ule after feeding, and we identified CYP74A187 (T107931_c2_g1), CYP94A140 (T107552_c0_g3), and CYP79A208 (T94418_c0_g1) as the three hub genes for the expression module. An additional nine key P450 genes were verified by qPCR. The jasmonic acid (JA) pathway key synthetase gene AOS (CYP74A187) was cloned, homology modeled, and subjected to molecular docking analysis. Conclusion This study identified the P450 gene family members of london planetree, and its expression, evolution, and anti-insect defense response. These data increased our understanding of the bio-logical stress of london planetree.