Atresia Ani: A Congenital Defect and Its Successful Management in Non-Descript Calf

Prakashkumar Chauhan, Vasantbhai Parmar, Tejasbhai Patel, Ketanbhai Thakor
2011 International Journal for Agro Veterinary and Medical Sciences  
Excretory /Secretory (E/S) antigen from Haemonchus contortus was isolated by culturing live adult worms in RPMI 1640 medium at 37 0 C for 24 hours and the culture supernatant was used as antigen. On immunoblot analysis, it was observed that the E/S antigen showed five reactive bands at 24, 29, 46, 66 and 93 kDa. On immunization trial, sheep were immunized with 500 µg of E/S antigen along with montanide as adjuvant on day 0, 30 and 60 intramuscularly. Further, the assessment of serum antibody
more » ... els in immunized sheep was made by enzyme linked immunosorbent assay (ELISA). Based on absorbance values, it was observed that the serum antibody levels were significantly (P≤0.01) higher up to 20 weeks post immunization in immunized sheep compared to control sheep.
doi:10.5455/ijavms.11977 fatcat:hx4vmaukzrfjhgtzwosv4zp24i