Lack of Peroxisomal Catalase Causes a Progeric Phenotype inCaenorhabditis elegans

Oleh I. Petriv, Richard A. Rachubinski
2004 Journal of Biological Chemistry  
Studies using the nematode Caenorhabditis elegans as a model system to investigate the aging process have implicated the insulin/insulin-like growth factor-I signaling pathway in the regulation of organismal longevity through its action on a subset of target genes. These targets can be classified into genes that shorten or extend life-span upon their induction. Genes that shorten life-span include a variety of stress response genes, among them genes encoding catalases; however, no evidence
more » ... r, no evidence directly implicates catalases in the aging process of nematodes or other organisms. Using genetic mutants, we show that lack of peroxisomal catalase CTL-2 causes a progeric phenotype in C. elegans. Lack of peroxisomal catalase also affects the developmental program of C. elegans, since ⌬ctl-2 mutants exhibit decreased egg laying capacity. In contrast, lack of cytosolic catalase CTL-1 has no effect on either nematode aging or egg laying capacity. The ⌬ctl-2 mutation also shortens the maximum life-span of the long lived ⌬clk-1 mutant and accelerates the onset of its egg laying period. The more rapid aging of ⌬ctl-2 worms is apparently not due to increased carbonylation of the major C. elegans proteins, although altered peroxisome morphology in the ⌬ctl-2 mutant suggests that changes in peroxisomal function, including increased production of reactive oxygen species, underlie the progeric phenotype of the ⌬ctl-2 mutant. Our findings support an important role for peroxisomal catalase in both the development and aging of C. elegans and suggest the utility of the ⌬ctl-2 mutant as a convenient model for the study of aging and the human diseases acatalasemia and hypocatalasemia.
doi:10.1074/jbc.m400207200 pmid:14996832 fatcat:3no46x5smvgxnixtxbqjdcy7uq